Fig. 1

Recognition of HIV-1 Env proteins by 1E5. HEK 293T cells were transfected with plasmid expressing EGFP and Env proteins of subtypes A, AE, B and C (a) and those of a panel of CRF02_AG (b). At 48 h post-transfection, cells were stained with primary antibody. Then, cells were fluorescently labeled with an allophycocyanin (APC)-conjugated anti-human IgG secondary Ab. Histograms of APC in EGFP⁺ cells stained with 1E5, normal human globulin (NHG) as a negative control, and VRC01 as a positive control, are shown by a blue line, gray shading and a red line, respectively