Fig. 4

DDX5 acts independently of DDX17. a Cells were sequentially transfected with siDDX5A and then a second round of siRNA together with pLAI, with or without increasing concentrations of a DDX17 expressor. Supernatant was harvested after 48 h and used to infect TZM-bl cells. The graph shown is a representative of three independent experiments done in triplicate. Bars represent mean of triplicate samples ± SEM. b Knockdown and rescue was determined by western blotting for DDX5 and DDX17 expression. Statistical significance: **P < 0.01. See also Figure S4