Fig. 8

LEDGIN treatment in primary CD4⁺ T cells hampers reactivation. a Primary CD4⁺ T cells from six independent donors were infected with wild type NL4.3 virus in presence of LEDGIN CX014442 or raltegravir. Four days post infection samples for qPCR were harvested, remaining cells were washed and activated with 10 nM PMA and 10 µg/ml PHA. Viral p24 was measured in the supernatant 7 days post infection. Results for six independent donors are represented by different dots and the average is shown by the connecting line. All results were plotted relative to the DMSO control, which was set to ‘1’ in each experiment. b The number of integrated copies per cell with increasing concentration of CX014442, as determined 4 days post infection. c The number of integrated copies 4 days after infection in the presence of raltegravir. d 72 h after activation, the fold activation of cells treated with CX014442 was calculated as the ratio of viral p24 in the supernatant of activated cells compared to non-activated cells. e The fold increase in p24 upon activation of cells treated with raltegravir. Statistical significance was calculated by the Kruskal–Wallis test that compared each concentration of compound with the DMSO control (*p < 0.05, **p < 0.01, ***p < 0.001). DMSO dimethyl sulfoxide, PMA phorbol 12-myristate 13-acetate, PHA phytohaemagglutinin