Fig. 2

Results of cDNA microarrays to examine subgroup-specific Tax or HBZ-mediated transcriptional changes in Jurkat Tet-On human T-cells. After transfection, Tax-A, Tax-B, HBZ-A or HBZ-B proteins were induced in Jurkat Tet-On 3G cells by adding Dox (final concentration: 3 ng/ml) for 24 h. Uninduced Jurkat Tet-On 3G cells were used as a baseline reference. Microarray was performed on RNAs derived from these samples. a The numbers of genes that occurred in each cluster are shown. We identified 231, 676, and 1712 genes that were specifically up- or down-regulated by Tax-A, by both Tax-A and Tax-B, and by Tax-B, respectively. In HBZ, 500, 731, and 6843 genes were specifically up- or down-regulated by HBZ-A, by both HBZ-A and HBZ-B, and by HBZ-B respectively. b Among 231, 676, and 1712 genes were specifically upregulated by HBZ-A, by both HBZ-A and HBZ-B, and by HBZ-B, respectively, 47, 62, and 410 genes were ncRNAs. Similarly, among 500, 731, and 6843 genes that were specifically upregulated by Tax-A, by both Tax-A and Tax-B, and by Tax-B, respectively, 167, 241, and 1245 genes were ncRNAs