Fig. 5
From: RNA-induced epigenetic silencing inhibits HIV-1 reactivation from latency
Cell viability during LRA treatment. The percentage of live cells was measured via negative gating of dead cells using the LIVE/DEAD fixable dye. These live cells were further gated to measure the GFP expression levels shown in Figs. 2 and 3, 10,000 live events were collected. a TNF, b SAHA, c SAHA and TNF, d Bryostatin and TNF, e Chaetocin, f DZNep. All treatments were assessed at 48 h, except from Chaetocin and DZNep, which were assessed at 72 h. The clinically relevant concentrations of TNF and SAHA are indicated in the corresponding plots. Comparisons shown were performed against the Parental cell line using the Kruskal–Wallis Multiple comparison test, correcting for Multiple comparison with Dunn’s test (*p ≤ 0.05)(Mean ± SD). Data shown is from at least one independent representative experiment performed in triplicates