Fig. 7

NF-κB elements are essential for RelB to enhance Tat recruitment to the LTR. a–d HeLa cells (1 × 10⁷) were transfected with pHIV-1-LTR-luc (6 μg) or pHIV-1-LTR mutNF-κB (6 μg), together with pFlag-Tat (6 μg) or vector plasmids. The relative expression of Tat and RelB in the transfected cells was monitored by western blots (a). Chromatin of the fixed cells was immunoprecipitated with the indicated antibodies. Samples were assessed for enrichment of HIV-1 LTR DNA by PCR (b). Tat (c) and RNA Pol II (d) associated HIV-1 LTR DNA was quantified by real-time PCR. (E to H) Control and RELB^−/− HeLa cells (1 × 10⁷) were transfected with pHIV-1-LTR mutNF-κB (6 μg), together with pFlag-Tat (6 μg) or vector plasmids (e). Samples were assessed for enrichment of HIV-1 LTR DNA by PCR. f Tat (g) and RNA Pol II (h) enriched HIV-1 LTR DNA was quantified by real-time PCR