Fig. 6
From: Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression
The κB elements are required for RelB to enhance Tat-mediated transcription of HIV-1 LTR. a Schematic representation of 5′-truncated pGL3-HIV-1-LTR-deletion reporter constructs. TAR (+ 1 to + 60), SP1 (− 79 to − 47), NF-κB (− 104 to − 81), and NRE (− 340 to − 184) sites are indicated. b Responsiveness of 5′-truncated pGL3-HIV-1-LTR reporter constructs to Tat and RelB. HEK 293T cells (0.2 × 106) were cotransfected with each of the 5′-truncated pGL3-HIV-1-LTR-deletion reporter constructs (100 ng) in the presence of Tat-expressing plasmids, together with pCMV-RelB constructs or control vectors. c HeLa cells (0.1 × 106) were transfected with the indicated HIV-1 LTR-Luc reporter plasmids (100 ng) and Tat-expressing plasmids (100 ng), together with pCMV-RelB constructs (100 ng) or control vectors. To normalize transfection efficiency, phRluc-TK (1 ng) was co-transfected. Luciferase assays were performed 48 h post transfection. The Rel. Luc. Act in the figure was calculated relative to normalized luciferase activity of pHIV-1-LTR-luc with Tat (with or without RelB) and vectors co-transfected cells