Table 3 Primers used for RNA in vitro binding assays and locked nucleic acid (LNA) sequences
From: Balanced splicing at the Tat-specific HIV-1 3′ss A3 is critical for HIV-1 replication
RNA substrate | Primer | Sequence |
|---|---|---|
Forward | #4759 | 5′ TAA TAC GAC TCA CTA TAG GG AC ATG AGG ATC ACC CAT GTG AAT TCG AAT AGG CGT TAC TCG ACA 3′ |
Reverse | #4761 | 5′ GGA TGC TTC CAG GGC 3′ |
LNA | Specifity | Sequence |
αESEtat | ESEtat | 5′ TTCTTGCTCTCCTCTG 3′ |
αESE2/ESS2 | ESE2/ESS2 | 5′ TCTAGTCTAGGATCTA 3′ |
Scrambled | - | 5′ GACGCGTCCTTACGCG 3′ |