Figure 1
From: The CRISPR/Cas9 system inactivates latent HIV-1 proviral DNA
Illustration of the ten HIV-1 guide RNAs tested in this study. (A) Locations of the 10 guide RNAs (T1 to T10) in HIV-1 genome. (B) Schematic depiction of binding of the T1 gRNA guide sequence (20 nucleotides, underlined) to the HIV-1 DNA in the context of Cas9 (in yellow). The PAM (protospacer adjacent motif) is highlighted in green letters. The red arrow indicates the cleavage site by Cas9. (C) Sequences of the 10 target sites in the genome HIV-1 strain HXB2 (T1 to T10). Conservation of each target sequence in HIV database is presented. The CRISPR score of each gRNA was calculated using the program at http://www.genome-engineering.org.