Figure 2From: Lysine methylation of HIV-1 Tat regulates transcriptional activity of the viral LTRTransiently transfected and integrated LTR reporter systems exhibit increased transactivation in the absence of SETDB1. A) Transient transfection of the CAT assay is broken down as follows: Lane 1 indicates the negative control; Lanes 2–4 titration of Tat from 0.01, 0.1 and 1.0 ug to establish a range of activation; Lanes 5–10 are in the presence of 0.1 ug Tat as well as the indicated transfected siRNAs. B) TZM-bl cells containing an integrated LTR-Luc were transfected with siGFP, siSETDB1, siTIF-1, siG9a, and siHP1 in addition to Tat (0.1 ug) to initiate transcription. Confirmation of the knockdown of SETDB1 is shown in a Western blot below. Each transfection and luciferase assay was repeated at least three times.Back to article page