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Fig. 9 | Retrovirology

Fig. 9

From: A single G10T polymorphism in HIV-1 subtype C Gag-SP1 regulates sensitivity to maturation inhibitors

Fig. 9

Mutation in HIV-1 subtype C IndieC1 Gag-SP1 residue 10 increases sensitivity towards BVM analogs. a HEK-293T cells were transfected with the HIV-1 subtype C clones, IndieC1 WT and its mutants SP1:S9N, SP1:T10G and SP1:S9N/T10G. Cells were treated with 100 nM of BVM analogs CV-8611, CV-8612, CV-8613 or with DMSO only. The virion-associated CA and CA-SP1 were detected by immunoblotting. Quantification of % CA-SP1 relative to total CA + CA-SP1 is presented in the graphs. The gel image shown here is representative of three independent experiments. b TZM-bl cells were infected with p24 normalized HIV-1 subtype C viruses IndieC1 WT and its mutants SP1:S9N, SP1:T10G and SP1:S9N/T10G produced from MI treated HEK-293T cells for 48 h to measure infectivity. Quantitative data for levels of infectivity relative to the DMSO control-treated sample is shown. Error bars indicate standard deviations from three independent experiments. The asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and ns indicates not significant

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