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Fig. 2 | Retrovirology

Fig. 2

From: Alpha-enolase in viral target cells suppresses the human immunodeficiency virus type 1 integration

Fig. 2

Effects of ENO1 knockdown in HIV-1 target cells on postentry step. a Flow cytometry monitoring of HIV-1 receptor CD4 and coreceptors CCR5 and CXCR4. Cell stained with isotype control antibody are indicated as negative control (black line). The expression levels of CD4, CCR5 and CXCR4 in control-siRNA (red line) or ENO1-specific-siRNA (blue line) -treated TZM-bl are shown. The expression level of each protein was examined just before the cells were infected with the virus. b Entry efficiency of HIV-1LAV-1 in either control or ENO1-knockdown TZM-bl cells. Entry efficiency was assessed on the basis of the amount of p24 from the cytosolic fraction of TZM-bl cells. The entry efficiency in the control-siRNA-treated TZM-bl cells was set as 100%. c Effect of ENO1 knockdown in TZM-bl cells on viral reverse transcription. The amount of R/gag products of viral reverse transcription was determined by quantitative real-time PCR analysis. d Effect of ENO1 knockdown in TZM-bl cells on viral cDNA nuclear import. The amount of 2-LTR circle products was determined by quantitative real-time PCR analysis. e Integration efficiency of HIV-1LAV-1 in either control or ENO1-knockdown TZM-bl cells. Relative amount of Alu-gag products was determined by nested-PCR. The integration efficiency in the control-siRNA-treated TZM-bl cells was set as 100%. Data are mean values ± SE from triplicate tests. The significance of difference (Student’s t-test) is indicated as follows: *, p < 0.05; n.s., not significant

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