Fig. 4

Expression of Tax and the NF-κB deficient Tax mutant M22 is fostered by induction of NF-κB with TPA/ ionomycin. a–c Jurkat T-cells were transiently transfected with EF1-α-driven Tax expression plasmids Tax (wildtype; 20 µg), M22 (NF-κB-deficient) or mock (100 µg each), replenished, where necessary, to 100 µg with the empty vector. At 24 h after transfection, cells were treated with 20 nM 12-O-Tetradecanoylphorbol-13-Acetate (TPA) and 1 µM ionomycin or the solvent control DMSO for another 24 h. a Immunoblot shows Tax, NF-κB2 p100/p52 and GAPDH. Numbers depict a representative densitometric analysis of Tax protein expression levels normalized on GAPDH. b Densitometric analysis of Tax protein expression relative to GAPDH, of four independent experiments was performed. Values ± SE are depicted and were compared using student’s t-test (*p < 0.05; **p < 0.01). c mRNA of Tax was measured by quantitative PCR (qPCR). Mean relative copy numbers (rcn), normalized on β-actin (ACTB) and on values derived from the DMSO solvent control, of four independent experiments ± SE are shown and were compared using student’s t-test (*p < 0.05; **p < 0.01)