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Fig. 2 | Retrovirology

Fig. 2

From: HTLV-1 CTCF-binding site is dispensable for in vitro immortalization and persistent infection in vivo

Fig. 2

Characterization of HTLV-1 proviral clones. An HTLV-1 LTR-luciferase assay and ELISA specific for HTLV-1 structural protein p19 Gag were performed as in vitro functional assays. HEK293T cells were co-transfected with an LTR-1-Luc reporter construct and 1 µg of HTLV-1, HTLV-1∆CTCF, HTLV-1p12Stop, or empty (pcDNA3) plasmids. 48 h post-transfection cells and supernatant were collected for LTR-transactivation luciferase assay a and p19 Gag ELISA b, respectively. HTLV-1, HTLV-1∆CTCF, HTLV-1p12Stop and proviral clones generated comparable LTR-transactivation and p19 Gag production

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