Figure 4

Neutralization activity of patient sera. (A) XMRV infected 293T cells (XMRV+ control) and XMRV-infected and treated with anti-XMRV sera at a dilution of 1:10 [14] are shown. (B) Data from non-XMRV infected 293T cells is shown as a control. Patients samples which did not show positive neutralization reaction (Patients with non-prostate cancer (NonPCa)-1, Patients with prostate cancer (PCa)-1) are shown. (C) Two samples that showed positive reaction from patients with prostate cancer (PCa-2 and -3) are shown. (D) Six samples that showed positive neutralization reaction from patients with non-prostate cancer (NonPCa-2 to -6) are shown. 1:10 dilution of sera were applied for all the experiments. Percent GFP positive and percent neutralization are indicated within the gated areas and below the flow data, respectively. The percent neutralization was calculated as the reciprocal of infectivity, with a maximum infectivity being determined by incubation of the virus with an uninfected mouse serum. n.a., not applicable.