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Figure 6 | Retrovirology

Figure 6

From: BLV-CoCoMo-qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm

Figure 6

Correlation between proviral load calculated by BLV-CoCoMo-qPCR and serial dilution nested PCR. (A) BLV proviral copy numbers for 1 μg of genomic DNA from 6 BLV-infected cattle were determined by BLV-CoCoMo-qPCR and serial dilution-nested PCR. For serial dilution-nested PCR, the 6 genomic DNAs were analyzed by serial tenfold dilution and subjected to nested PCR for detection of the BLV LTR gene. Nested PCR reactions were repeated 10 times and proviral load was calculated according to a Poisson distribution model as shown in Methods. Values represent the mean ± standard deviation (SD) of results from four independent experiments. (B) Scatter chart is indicated the correlation between BLV copy numbers which were determined by BLV-CoCoMo-qPCR and by serial dilution-nested PCR.

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