Figure 5
From: BLV-CoCoMo-qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm
Comparison of the sensitivity of BLV-CoCoMo-qPCR and nested PCR. Ten samples containing 0.7 copies of pBLV-LTR/SK were amplified by nested PCR (A) and real-time PCR with the CoCoMo 6 and CoCoMo 81 primer set (B). The 168-bp band was used to detect BLV LTR amplicons (A). Carboxy-X-rhodamine (ROX) intensities were used for corrections of tube differences, and carboxyfluorescein (FAM) intensities were used to detect BLV LTR amplicons (B).