HIV-1 nef suppression by virally encoded microRNA

Retrovirology

Table 1 Relation between AIDS clinical courses and nef dsRNA or siRNA/miRNA in suppression of nef gene expression

Nef inhibitor	Nef expressed by:			Target region	Clinical courses
	Nef-EGFP	HIV-1 IIIB	PFV/nef
*dsRNA* *					*Human*
SF2	++ ^†	++	ND^‡	Full-length (including miR-N367)	ND
1-3-3	++	-	ND	U3 deleted	Rapid progressor, Died within 3 years
4-2-1	ND	+	ND	U3 region (including miR-N367)	Rapid progressor, Died within 3 years
15-2-2	++	+++	ND	U3 region (including miR-N367)	Non progressor with low plasma viremia
16-1-1	ND	+	ND	U3 deleted	Non progressor with low plasma viremia
jw95-1	ND	+	ND	U3 region (including miR-N367)	Non progressor with undetectable viremia
*siRNA*					*15-2-2 model mouse*
dsnef	+	+	+	Full-length	ND
sinef007	+	ND	ND	Upstream of U3 region	ND
sinef084	+	ND	ND	Upstream of U3 region	ND
sinef176	+++	+++	++	Upstream of U3 region^§	ND
sinef190	+++	+++	+++	Upstream of U3 region	ND
sinef299	+	ND	ND	U3 region (aa 83–135)	ND
sinef367/miR-N367	++	+++	++	U3 region	No weight loss
sinef468	+	ND	ND	U3 region	ND
sinef580	+	ND	ND	U3 region	ND

*dsRNA to LTNPs' nef has been described (Yamamoto et al., 2002).
^† -, negative; +, 0 to 50 % inhibition; ++, 50 to 75% inhibition; +++, 75 to 100 % inhibition.
^‡ ND, not done.

ISSN: 1742-4690